Hemin-deficient mutants of Escherichia coli K-12

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Hemin-deficient mutants of Escherichia coli K-12.

The occurrence of hemin-deficient bacterial mutants became known in 1953, when Jensen and Thofern (3) were able to isolate, by using streptomycin, a hemin-requiring mutant of Staphylococcus aureus. By their ability to grow as "small colony variants" (SCV), forming dwarf colonies in the absence of hemin, these mutants differ sharply from genuine conditioned lethal auxotrophic mutants, and resemb...

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Lipopolysaccharide-deficient, bacteriophage-resistant mutants of Escherichia coli K-12.

Bacteriophage-resistant mutants isolated and classified in a previous study were examined for alterations in their lipopolysaccharide (LPS) composition, and properties likely to be affected by alterations in LPS composition were studied. It was found that many of the mutants of the Ktw (K2-resistance), Ttk (T2, T4, or K19 resistance), Bar (bacteriophage), Wrm (wide-range mutants), and miscellan...

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Phosphoglucomutase mutants of Escherichia coli K-12.

Bacteria with strongly depressed phosphoglucomutase (EC 2.7.5.1) activity are found among the mutants of Escherichia coli which, when grown on maltose, accumulate sufficient amylose to be detectable by iodine staining. These pgm mutants grow poorly on galactose but also accumulate amylose on this carbon source. Growth on lactose does not produce high amylose but, instead, results in the inducti...

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Isolation and characterization of conjugation-deficient mutants of Escherichia coli K-12.

Conjugation-deficient mutants (Con-) of Escherichia coli K-12 have been isolated by a variety of indirect selective techniques. Mutants with mutations conferring ampicillin resistance, fosfomycin resistance, an alanine requirement, and a failure of ferment a number of carbohydrates were selected because the impaired functions occur in association with cell wall and cell membrane defects. The in...

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Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG.

ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introduced into ruvA, ruvB, and ruvC strains. Conjugational recombination was reduce...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1968

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.96.2.570-572.1968